General Cell Based Assays
Cayman Chemical GlycolysIs Cel-Based asy K 1ea
Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase (LDH) catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between 490 and 520 nm. The quantity of formazan produced is proportional to the quantity of lactate in the culture medium and is thus an indirect measurement of glycolysis. This assay can be adapted to high-throughput screening to examine glycolytic regulators in a cell-based system. In addition, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers.
MARSHALL SCIENTIFIC Gilson Classic Large Volume Kit
The PIPETMAN Classic line is one of the world standards in pipetting and offers a wide range of continuously adjustable, air-displacement pipettes with legendary accuracy and precision. The PIPETMAN Classic Large-Volume Kit provides two pipettes that cover large volume transfers from 500 uL to 10 mL, along with appropriate filters. Made of highly resistant materials like PVDF and stainless steel, PIPETMAN Classic offers uncontested durability and reliability. Built-to-last, it is the economical solution for years of pipetting.
Indigo Biosciences Human FGFR4/β-Klotho Reporter Assay Kit, 1 x-96 well format assays
Human FGFR4/β-Klotho Reporter Assay Kit, 1 x-96 well format assays
LABSYSTEMS DIAGNOSTICS OY Immunosuppressants CP DIRECT KIT-500 ASSAYS
Immunosuppressants CP DIRECT KIT-500 ASSAYS
Cayman Chemical HDAC CelBased ActIvIty96
Cayman’s HDAC Cell-Based Assay Kit provides a robust and EAsy-to-use platform for studying HDAC activity modulators in whole cells. By using a cell-permeable HDAC substrate, the activity of various lysine-specific protein deacetylases can be measured in intact cells in a simple and homogenous manner. The fluorescence of the deacetylated reaction product can be analyzed using a fluorometer with an excitation wavelength of 340-360 nm and an emission wavelength of 440-460 nm. An HDAC inhibitor, trichostatin A, is included for checking specificity of the HDAC reaction. This assay parallels Cayman’s HDAC Activity Assay Kit (Item No. 10011563), which uses a nuclear extract rather than whole cells for the assay. Together, both assays will help identify potential inhibitors/activators of HDAC enzymes.
Aat Bioquest Annexin V-iFluor 555 conjugate (100 tests)
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. This catalog is for 100 tests.
Eurofins Discoverx PathHunter eXpress GPR107 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0419E2ACP1M)
The PathHunter GPR107 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR107 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR107 and EA-tagged β-Arrestin-2. Activation of GPR107 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
STEMCELL Technologies Phalloidin iFluorTM 647
Fluorescent dye conjugates for detection of F-actin in cell culture experiments - 300 Tests
Cayman Chemical Catlase asy KIt 96Wel
Catalase is an ubiquitous antioxidant enzyme that is present in most aerobic cells. It is involved in the detoxification of H2O2, a ROS that is a toxic product of both normal aerobic metabolism and pathogenic ROS production. Cayman’s Catalase Assay utilizes the peroxidatic function of catalase for determination of enzyme activity. The assay can be used to measure catalase activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates.
Cayman Chemical EfferocytosIs asy KIt 1ea
Cayman’s Efferocytosis Assay Kit employs CytoTell™ Blue to visualize effector (phagocytic) cells, CFSE to visualize bait (apoptotic) cells and staurosporine, a protein kinase C inhibitor, to induce apoptosis. The Efferocytosis Assay Kit is a convenient tool to study modulators of efferocytosis, apoptosis, pro-resolving mediators and cross-presentation. The reagents provided in this kit are sufficient to stain 5 x 107 effector cells and 2 x 108 bait cells.
Cayman Chemical NOS ActIvIty asy KIt 1ea
The NOS Activity Assay measures NOS activity by monitoring the conversion of radiolabeled arginine to citrulline. This assay is simple, sensitive, and specific for NOS activity and can be used with both crude and purified enzyme preparations. The kit includes sufficient materials and reagents for 50 total reactions. Radiolabeled arginine and NADPH are not included with the kit.